Figure 1.

Sequences of Cotton leaf curl Multan virus (CLCuMV) used to produce hairpin constructs. The sequences used to produce hairpin constructs are highlighted in red on the genome map of CLCuMV. The virus-encoded genes and their orientation are indicated as arrows. The genes encode the V2 protein, the coat protein (CP), the replication-associated protein (Rep), the transcriptional activator protein (TrAP), the replication enhancer protein (REn) and the C4 protein. The origin of virion-sense DNA replication is indicated by the black dot at position zero. Sequence I consisted of the overlapping Rep and C4 gene sequences which was used to produce construct CLCRNAiRepC4/pFGC. Sequence II consisted of the overlapping Rep, TrAP and REn gene sequences and was used to produce construct CLCRNAiRepTrAPREn/pFGC. Sequence III contained sequence derived from the intergenic region and was used to make construct CLCRNAiIR/pFGC.