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. 2012 Apr 1;23(7):1208–1218. doi: 10.1091/mbc.E11-08-0723

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© 2012 Ydenberg et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 1: — The Dbl-homology domain is required for cell fusion. (A) Fus2p-ΔDbl-GFP localizes to the shmoo tip. Cells (MY9181) transformed with a plasmid expressing either Fus2p-GFP (pMR5469) or Fus2p-ΔDbl-GFP (pMR5883) were treated with pheromone as indicated for 2 h and examined. (B) Fus2p-ΔDbl-GFP is expressed at similar levels as the wild-type Fus2p-GFP protein. The same strains as in A were analyzed by Western blot using anti-GFP. (C) Diploid formation is defective in fus2-ΔDbl. Wild type (DDY1300), fus2Δ (MY10016), and fus2-ΔDbl (MY10933) were mated to fus1 fus2 (MY10798) for 4 h at 30°C. (D, E) Cell fusion is defective in fus2-ΔDbl. The same strains as in C were mated to fus2 (MY10797) for 2 h, washed into azide, and stained with FM4-64 to label the plasma membrane. (D) Examples of fusion-defective zygotes. Wild-type zygote (DDY1300xMY10797), partial Fus⁻ zygote (MY10933xMY10797), and full Fus⁻ zygote (MY10933xMY10797). (E) Percentage of zygotes of each morphology produced in matings with the indicated genotype. Gray bars, partial Fus⁻ zygotes; black bars, full Fus⁻ zygotes. n ≥ 133 zygotes imaged in two independent experiments.