FIGURE 6:
Fus2p and Cdc42p work in the same pathway. (A) Cytoplasmic transfer assay. Strains of different genotypes were mated to MY10797 (fus2 PGPD1-GFP) for 2 h at 30°C. An example of a zygote displaying cytoplasmic transfer (top) and one not displaying transfer (bottom) are shown. (B) WT (DDY1300), cdc42-138 (DDY1354), fus2 (MY10016), and fus2 cdc42-138 (MY10310) were mated to MY10797, and the percentage of zygotes showing transfer were counted. The total number of zygotes counted in three independent experiments are shown.