TABLE 1:
Strains used this study.
| Strain | Genotype | Reference |
|---|---|---|
| JY428 | α fus2Δ3 his4-34 trp1Δ1 ura3-52 | G. Fink (Whitehead Institute, Cambridge, MA) |
| JY429 | α fus1Δ1 fus2Δ3 trp1Δ1 ura3-52 | G. Fink |
| JY431 | α his4-34 trp1Δ1 ura3-52 | G. Fink |
| DDY1300a | a ura3-52 leu2-3112 his3Δ200 lys2-801 CDC42::LEU2 | Kozminski et al. (2000) |
| DDY1301 | α ura3-52 leu2-3112 his3Δ200 lys2-801 CDC42::LEU2 | Kozminski et al. (2000) |
| DDY1354 | a ura3-52 leu2-3112 his3Δ200 lys2-801 cdc42-138::LEU2 | Kozminski et al. (2000) |
| DDY1355 | α ura3-52 leu2-3112 his3Δ200 lys2-801 cdc42-138::LEU2 | Kozminski et al. (2000) |
| MY9181 | a ura3Δ0 leu2Δ0 his3Δ1 met15Δ0 fus2Δ::HIS3 | Paterson et al. (2008) |
| MY10016 | a ura3-52 leu2-3112 his3Δ200 lys2-801 CDC42::LEU2 fus2Δ::HIS3 | This study |
| MY10303 | α ura3-52 leu2-3112 his3Δ200 ade2-1 fus2Δ::HIS3 | This study |
| MY10310 | a ura3-52 leu2-3112 his3Δ200 lys2-801 cdc42-138::LEU2 fus2Δ::HIS3 | This study |
| MY10547 | a ura3-52 leu2-3112 his3Δ200 lys2-801 cdc42-V36M::LEU2 | This study |
| MY10554 | α ura3-52 leu2-3112 his3Δ200 ade2-1 cdc42-V36M::LEU2 | This study |
| MY10797 | α fus2Δ3 his4-34 trp1Δ1 ura3-52 SSO1::PGPD1-GFP::URA3 | This study |
| MY10798 | α fus1Δ1 fus2Δ3 trp1Δ1 ura3-52 SSO1::PGPD1-GFP::URA3 | This study |
| MY10925 | a ura3-52 leu2-3112 his3Δ200 lys2-801 CDC42::LEU2 STE20ΔCRIB | This study |
| MY10926 | a ura3-52 leu2-3112 his3Δ200 lys2-801 cdc42-138::LEU2 STE20ΔCRIB | This study |
| MY10933 | a ura3-52 leu2-3112 his3Δ200 lys2-801 CDC42::LEU2 fus2ΔDbl::GFP | This study |
| MY12402 | a ura3-52 leu2-3112 his3Δ200 pep4::HIS3 bar1::kanMX fus2::natMX | This study |
aStrains containing cdc42-105, cdc42-107, and cdc42-137 are isogenic to DDY1300 (or DDY1301). See Kozminski et al. (2000).