Figure 3.

Relative specific activity of β-galactosidase from cells not treated or treated with ponasterone A. These cell lines were permanently transfected with the nonmethylated or the demethylated reporter gene. In the latter, the cell line was further stably transfected with an empty vector or a vector containing 5-MCDG or a mutant of 5-MCDG. Cells treated with 10 μM ponasterone A were tested for β-galactosidase activity 48 h later. ONPG, ortho-nitrophenyl-β-D-galactopyranoside.