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Western blot of nuclear extract with anti-5-MCDG (G⋅T mismatch glycosylase) antibodies. (Upper) Lanes: 1, nuclear extract from parent cells (293 M+); 2, nuclear extract from parent cells transfected with the inactive mutant of 5-MCDG; 3, nuclear extract from cells transfected with the empty vector; 4, nuclear extract from cells transfected with 5-MCDG cDNA. (Lower) Total protein loading with Ponceau staining before immunoreaction.
