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Activity of 5-MCDG from nuclear extracts. Average values (triplicates) obtained by cutting out the specific band (27-mer) from the gel and measuring the radioactivity. (Inset) Separation of the reaction product (27-mer) on a sequencing gel (20% polyacrylamide). Lanes: 1, parent cell line; 2, cell line transfected with the empty vector; 3, cell line transfected with the inactive mutant of 5-MCDG; 4, cell line transfected with the 5-MCDG; 5, blank.
