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. 2001 Apr 10;98(9):5031–5036. doi: 10.1073/pnas.091097298

Figure 6.

Figure 6

Southern blot of genomic DNA from clones of stably transfected kidney cells digested as follows. Lanes: D, BglII and AflII; M, BglII, AflII, and MspI; H, BglII, AflII, and HpaII. Vc2 and Vc3 are clones stably transfected with the empty vector. HGc2, HGc5, and HGc7 are clones stably transfected with 5-MCDG. (These last three clones stain positive for β-galactosidase activity after ponasterone A treatment). When the promoter-enhancer is demethylated, digestion with HpaII gives DNA fragments of 180–300 bp.