FIGURE 4:
Repression of GAL1 is delayed with a disrupted gene–periphery tether. Wild-type, nup1Δ, and ada2Δ strains were grown at room temperature in YP plus 2% raffinose to mid-log phase. GAL1 mRNA expression was induced by galactose addition for 2 h and then inhibited by the addition of glucose for the indicated time. GAL1 mRNA levels were monitored by qRT-PCR and normalized to the control gene, ACT1. The fold change is calculated relative to the transcript levels for each strain at the zero time point, defined as 1.0.