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. 2012 Apr 1;23(7):1367–1375. doi: 10.1091/mbc.E11-06-0547

FIGURE 4:

FIGURE 4:

Repression of GAL1 is delayed with a disrupted gene–periphery tether. Wild-type, nup1Δ, and ada2Δ strains were grown at room temperature in YP plus 2% raffinose to mid-log phase. GAL1 mRNA expression was induced by galactose addition for 2 h and then inhibited by the addition of glucose for the indicated time. GAL1 mRNA levels were monitored by qRT-PCR and normalized to the control gene, ACT1. The fold change is calculated relative to the transcript levels for each strain at the zero time point, defined as 1.0.

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