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. 2012 Apr 1;23(7):1146–1156. doi: 10.1091/mbc.E11-07-0624

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© 2012 De Smet et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 4: — Co-overexpression of Ole1p and Sct1p reveals competition for the shared substrate C16:0-CoA. (A) sct1pct1 cells transformed with the indicated plasmids were cultured to mid-log phase in SGR and analyzed for their fatty acid contents by gas chromatography. The relative abundance (mol%) of the six major fatty acids and of total saturated fatty acids is shown, with the error bars representing the variation (n = 2). (B) Western blot analysis of the Ole1p expression levels of sct1pct1 pYES2 + pESC-OLE1 and sct1pct1 pYES2-SCT1-HH + pESC-OLE1. (c) Serial dilutions of sct1pct1 cells transformed with the plasmids indicated and precultured in synthetic glucose medium were spotted on SGR plates and incubated at 30°C for 3 d.