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. Author manuscript; available in PMC: 2012 Mar 30.
Published in final edited form as: Protein Expr Purif. 2008 Sep 20;63(2):102–111. doi: 10.1016/j.pep.2008.09.008

Fig. 1. Multiple cloning sites of pEHISTEV /pEHISGFPTEV.

Fig. 1

Both vectors were constructed containing the f1 replication origin, kanamycin resistance gene, pB332 DNA replication origin, and lac repressor gene. The sequence details of the cloning/expression region show the T7 promoter, lac operator, 6xHis-tag (pEHISTEV) or 6xHis-tagged GFP (pEHISGFPTEV), TEV protease recognition site, restriction edonuclease sites for cloning, and the T7 terminator.