Both vectors were constructed containing the f1 replication origin, kanamycin resistance gene, pB332 DNA replication origin, and lac repressor gene. The sequence details of the cloning/expression region show the T7 promoter, lac operator, 6xHis-tag (pEHISTEV) or 6xHis-tagged GFP (pEHISGFPTEV), TEV protease recognition site, restriction edonuclease sites for cloning, and the T7 terminator.