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. Author manuscript; available in PMC: 2012 Mar 30.
Published in final edited form as: Protein Expr Purif. 2008 Sep 20;63(2):102–111. doi: 10.1016/j.pep.2008.09.008

Fig. 2. HisGFP mediated soluble expression screening.

Fig. 2

A). Colonies of E. coli cells transformed with pEHISGFPTEV constructs (top) expressing GFP-NFIIIhd, RanBP22532-2767, Mutase-kp or Sso2226 and with their pEHISTEV constructs (bottom) on the L-agar plate containing 0.4 mM IPTG. We found that the His-tagged and His-GFP colonies could be combined on the same plate and selection was still straightforward. B) Quantitative analysis of soluble expression of the target proteins under the optimised conditions. Measurement of OD488 of purified HisGFP is shown on the bottom and the extinction coefficient was calculated based on Beer’s Law. The concentration of the expressed target proteins (after His-GFP removal) was calculated as described in the Materials and Methods section.