Table 1.
Binding model | Predicted responsea | |
---|---|---|
Non-saturable interaction | mLapp = mL1Ka[D] | (1) |
Single group of saturable sites | mLapp = (mL1Ka1[D])/(1 + Ka1[D]) | (2) |
Two interactions, saturable site + non-saturable | mLapp = (mL1Ka1[D])/(1 + Ka1[D]) + mL2Ka[D] | (3) |
Two groups of saturable sites | mLapp = (mL1Ka1[D])/(1 + Ka1[D]) + (mL2Ka2[D])/(1 + Ka2[D]) | (4) |
Symbols: mLapp, moles of applied analyte required to reach the mean position of the breakthrough curve; mL1, total moles of active binding site 1; Ka1, association equilibrium constant for saturable binding of the analyte to the ligand at site 1; [D], concentration of the applied drug; mL2, total moles of active binding site 2; Ka2, association equilibrium constant for saturable binding of the analyte to the ligand at site 2; Ka, association equilibrium constant for the analyte in a non-saturable interaction.