. Author manuscript; available in PMC: 2013 Apr 1.

Published in final edited form as: Anal Bioanal Chem. 2012 Feb 22;403(2):563–571. doi: 10.1007/s00216-012-5816-y

Table 1.

Binding models used to fit frontal analysis data for R-propranolol and S-propranolol on LDL columns

Binding model	Predicted response^a
Non-saturable interaction	m_Lapp = m_L1K_a[D]	(1)
Single group of saturable sites	m_Lapp = (m_L1K_a1[D])/(1 + K_a1[D])	(2)
Two interactions, saturable site + non-saturable	m_Lapp = (m_L1K_a1[D])/(1 + K_a1[D]) + m_L2K_a[D]	(3)
Two groups of saturable sites	m_Lapp = (m_L1K_a1[D])/(1 + K_a1[D]) + (m_L2K_a2[D])/(1 + K_a2[D])	(4)

Symbols: m_Lapp, moles of applied analyte required to reach the mean position of the breakthrough curve; m_L1, total moles of active binding site 1; K_a1, association equilibrium constant for saturable binding of the analyte to the ligand at site 1; [D], concentration of the applied drug; m_L2, total moles of active binding site 2; K_a2, association equilibrium constant for saturable binding of the analyte to the ligand at site 2; K_a, association equilibrium constant for the analyte in a non-saturable interaction.