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Structural determinants of RIα required for its NMJ enrichment. Percentage of coincidence of tetramethylrhodamine B isothiocyanate-α-bungarotoxin and GFP fluorescence was obtained with plasmids encoding full-length RIα(pRIα-GFP), N-terminal sequences of RIα(residues 1–91 or 1–81) or of RIIα (residues 1–44), and single amino acid substitutions of full-length RIα (C18A, V22A, I27A, F54A). The number of GFP-positive fibers examined for each construct is indicated in parentheses.
