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. 2012 Feb 29;5:10. doi: 10.1186/1754-6834-5-10

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Copyright ©2012 Qu et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Southern blot analysis of T0 and T1 plants from X8-FAD2-1i #34 line. (A) Total genomic DNA were digested with XhoI and hybridized with soybean 7S promoter probe. *Shows the positive genomic bands containing marker; **shows the positive genomic bands that are marker free. (B) Total genomic DNA were digested with EcoRV and XbaI and hybridized with FAD2-1 probe (left panel). The same membrane was stripped and hybridized with HPT probe (right panel). The signals shared between wild-type and the transgenic lines represent the endogenous JcFAD2-1 sequence. Each of the transgenic lines also shows one extra band corresponding to the transgene insert.