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. 2012 Mar 27;443(Pt 2):485–490. doi: 10.1042/BJ20111057

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© 2012 The Author(s) The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Non-Commercial Licence (http://creativecommons.org/licenses/by-nc/2.5/) which permits unrestricted non-commercial use, distribution and reproduction in any medium, provided the original work is properly cited.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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(A) SDS/PAGE of pAp-affinity chromatography from HeLa cell nuclear extracts. The gel was stained with Colloidal Blue. Lane 1, pAp-binding fraction; lane 2, binding on control agarose beads; lane 3, molecular mass markers. (B) PARP-1-affinity chromatography with AMP-agarose or pAp-agarose. Partially purified PARP-1 (1 μg) was incubated with AMP-agarose (lanes 1–4) or pAp-agarose (lanes 5–8) in the presence (+) or absence (−) of 3 mM pAp. Detection of PARP-1 in the bound (B) or unbound (U) fractions was performed by Western blot analysis. Lane 9, PARP-1 input.