FIG. 4.

Real-time PCR analysis of stromelysin mRNA expression in rat theca-interstitial cells cultured in vitro for Mmp3 (A), Mmp10 (B), and Mmp11 (C). Cells were collected from eCG-primed immature rats and cultured in the absence (Control) or presence of FSK + PMA for varying lengths of time. Relative levels of mRNA for each stromelysin member were normalized to Rpl32 for each sample. Values represent the mean ± SEM (n = 3–4 animals/time point). Bars with no common superscripts in each panel are significantly different (P < 0.05).