Skip to main content
. 2011 Dec 21;86(3):96. doi: 10.1095/biolreprod.111.097030

FIG. 5.

FIG. 5.

Dexra prevented DXR-induced DNA damage in stroma/theca and granulosa cells from in vitro cultured murine ovaries and primary murine granulosa cells. Bar graphs summarize the OM quantification from four experiments (*P < 0.05, one-way ANOVA). Example images are given to the right of each graph. In vitro cultured ovaries or primary granulosa cells were pretreated with 20 μM Dexra or DMSO (the vehicle carrier control) followed by the addition of DXR for 3 h in the continued presence of Dexra. A) Stroma/theca cells: Dexra prevented or reduced the 45%–65% increase in DNA damage induced by 50 nM, 500 nM, or 10 μM DXR (vs. DMSO carrier for control) in stroma/theca cells from in vitro culture ovaries. B) Granulosa cells: Dexra eliminated the 45%–65% increase in DNA damage caused by exposure to 50 nM, 500 nM, or 10 μM DXR in granulosa cells from in vitro cultured ovaries. C) Oocytes: Oocytes from ovaries treated in vivo for 3 h with DXR did not show significant DNA damage response in the NCA (P > 0.05, one-way ANOVA), and the presence of 20 μM Dexra did not alter the comet moment (OM). D) Primary murine granulosa cell culture: Dexra eliminated the 20%–40% increase in DNA damage induced by 50 nM, 500 nM, or 10 μM DXR in primary murine granulosa cells. Original magnification ×200.