Fig. 2.

MR antagonism with Sp improves proximal tubule oxidative stress in the TG Ren2 (R2) rat. a Depicts representative images of semiquantitative immunohistochemistry analysis of NADPH oxidase (Nox) subunit Nox2 with corresponding measures of intensity to the right. b Representative images of 3-NT content, a marker of peroxynitrite formation (ONOO^–), with corresponding measures to the right. Values represented as mean ± SEM. ∗ p < 0.05 when Ren2 controls (R2-C) are compared to SD controls (SD-C); ∗∗ p < 0.05 when Sp treated Ren2s at low dose (R2-LSp) or conventional dose (R2-Sp) are compared to R2-C; ^‡ p < 0.05 when proximal tubule intensities are compared to glomerular intensities. Scale bar = 50 μm.