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. Author manuscript; available in PMC: 2012 Mar 30.
Published in final edited form as: Dev Dyn. 2006 Mar;235(3):701–710. doi: 10.1002/dvdy.20686

Fig. 3. Forkhead proteins transactivate Tbx1 in a dose dependent manner.

Fig. 3

Foxa2, Foxc1 or Foxc2 and Tbx1-luciferase reporter were cotransfected into COS-1 cells. The horizontal axis of bar graphs shows the relative fold of measured luciferase activity. (A) Fox proteins transactivate Tbx1 reporter with 200 bp fragment involving Fox site #1 (Fox #1-luc) as well as Tbx1 reporter with 200 bp involving Fox site #2 (Fox #2-luc). Both Fox site #1 with Fox mutation (Fox #1 mt-luc) and Fox site #2 with Fox mutation (Fox #2 mt-luc) abolish the activation. (B) 250ng to 750ng of expression plasmids of Fox proteins were cotransfected with Tbx1 reporter containing Fox site #2 (Fox #2-luc) and demonstrated transactivation in a dose dependent manner. (C) Increasing copies of 68 bp fragment of Fox site #1 using four (Fox #1 × 4-luc) or eight (Fox #1 × 8-luc) tandem repeats of Fox site #1 resulted in enhanced transactivation by Foxa2, Foxc1 and Foxc2 as the number of Fox site #1 increased.