Table 1. Intra- and inter-assay reproducibility of the GPL fatty acid analysis.
Intra-assay (nā=ā8) | Inter-assay (nā=ā26) | |||||
Mean | SD | CV [%] | Mean | SD | CV [%] | |
Saturated Fatty Acids | ||||||
C14:0 | 0.35 | 0.01 | 3.7 | 0.32 | 0.04 | 12.6 |
C16:0 | 22.99 | 1.01 | 4.4 | 23.31 | 0.80 | 3.4 |
C17:0 | 0.34 | 0.02 | 4.6 | 0.32 | 0.04 | 11.3 |
C18:0 | 18.34 | 0.27 | 1.5 | 17.92 | 0.40 | 2.3 |
Monounsaturated Fatty Acids | ||||||
C16:1n-7 | 0.29 | 0.02 | 7.6 | 0.35 | 0.03 | 8.6 |
C18:1n-7 | 1.45 | 0.03 | 2.0 | 1.42 | 0.15 | 10.9 |
C18:1n-9 | 14.07 | 0.22 | 1.5 | 13.38 | 0.22 | 1.6 |
C20:1n-9 | 0.29 | 0.00 | 3.3 | 0.27 | 0.01 | 4.9 |
n-9 Polyunsaturated Fatty Acids | ||||||
C20:3n-9 | 0.11 | 0.00 | 4.2 | 0.11 | 0.01 | 9.5 |
n-6 Polyunsaturated Fatty Acids | ||||||
C18:2n-6 | 11.84 | 0.50 | 4.3 | 12.34 | 0.16 | 1.3 |
C18:3n-6 | 0.06 | 0.00 | 3.7 | 0.08 | 0.01 | 10.1 |
C20:2n-6 | 0.22 | 0.01 | 6.4 | 0.25 | 0.01 | 5.3 |
C20:3n-6 | 2.37 | 0.02 | 1.0 | 2.21 | 0.04 | 1.7 |
C20:4n-6 | 15.39 | 0.84 | 5.5 | 15.57 | 0.55 | 3.5 |
C22:4n-6 | 2.37 | 0.25 | 10.5 | 3.20 | 0.20 | 6.3 |
C22:5n-6 | 0.67 | 0.02 | 3.0 | 0.64 | 0.03 | 5.0 |
n-3 Polyunsaturated Fatty Acids | ||||||
C18:3n-3 | 0.15 | 0.01 | 4.6 | 0.10 | 0.01 | 8.7 |
C20:5n-3 | 0.75 | 0.04 | 5.5 | 0.69 | 0.05 | 6.6 |
C22:5n-3 | 2.35 | 0.22 | 9.3 | 2.31 | 0.14 | 6.2 |
C22:6n-3 | 5.68 | 0.17 | 2.9 | 5.16 | 0.26 | 5.0 |
Mean and SD are expressed as %wt/wt.