Table 1. Primers used for PCR amplification in this study.
Gene | Primer | Primer sequence (5′- 3′) | Product size (bp) |
cox1 | UEA3 | TATAGCATTCCCACGAATAAATAA | UEA3-UEA8: 1016 |
cox1 | UEA8 | AAAAATGTTGAGGGAAAAATGTTA | |
cob | CBF1 | TATGTACTACCATGAGGACAAATATC | CBF1-CBR1: 485 |
cob | CBR1 | ATTACACCTCCTAATTTATTAGGAAT | |
rrnL | 16Sar | CGCCTGTTTAACAAAAACAT | 16Sar-16Sbr: 490 |
rrnL | 16Sbr | CCGGTCTGAACTCAGATCACGT | |
nad5 | N5-J7077 | TTAAATCCTTWGARTAAAAYCC | J7077-N7793: 733 |
nad5 | N5-N7793 | TTAGGTTGRGATGGNYTAGG | |
cox1 | P1 | GAGGGGGGGACCCAATTCTCTTTCAATATT | P1–P2: 3173 |
cob | P2 | GAAATGATAGGAATCGTGTTAGTGTGGGAT | |
cob | P3 | ATCCCACACTAACACGATTCCT | P3–P4: 5853 |
cox1 | P4 | ACATAGTGGAAGTGGGCAACAACAT | |
cox1 | P9 | CTATGTTGTTGCCCACTTCCACTATGTTC | P9–P10: 8098 |
cox1 | P10 | CCCCCTCCAGATGGATCAAAGAATGAAGTT | |
rrnL | P5 | TTAATTCAACATCGAGGTCGCAATCACAAG | P5–P6: 2099 |
nad5 | P6 | TGGAAGCCCCATTATTGAGCAT | |
nad5 | P7 | CTGGTGTGTATCTCCTCTATCG | P7–P8: 6244 |
rrnL | P8 | CTTGTGATTGCGACCTCGATGTTGAATTAA | |
nad4 | P11 | TTATGTTCGTGTCAAAACCCG | P11–P12: 7394 |
nad4 | P12 | TAGTCGTTCATACTGTTTCCCTC | |
cob | qcobF | TTGGGGTCGCTTCTCGGGCT | qcobF-qcobR: 201 |
cob | qcobR | TCCGACATGGGCGTAGCAGA | |
nad1 | qnad1F | TAGTATTATCCTTCTTTATTGGC | qnad1F- qnad1R: 157 |
nad1 | qnad1R | ACAATGGTTAGGGGGAGGTAGAT | |
Nuclear gene | β-ActinS | CACGGTATCGTCACCAACTG | β-ActinS-β-ActinA: 207 |
β-ActinA | AGACAATACGGCTTGGATGG |