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. 2012 Mar 30;7(3):e33973. doi: 10.1371/journal.pone.0033973

Table 1. Primers used for PCR amplification in this study.

Gene Primer Primer sequence (5′- 3′) Product size (bp)
cox1 UEA3 TATAGCATTCCCACGAATAAATAA UEA3-UEA8: 1016
cox1 UEA8 AAAAATGTTGAGGGAAAAATGTTA
cob CBF1 TATGTACTACCATGAGGACAAATATC CBF1-CBR1: 485
cob CBR1 ATTACACCTCCTAATTTATTAGGAAT
rrnL 16Sar CGCCTGTTTAACAAAAACAT 16Sar-16Sbr: 490
rrnL 16Sbr CCGGTCTGAACTCAGATCACGT
nad5 N5-J7077 TTAAATCCTTWGARTAAAAYCC J7077-N7793: 733
nad5 N5-N7793 TTAGGTTGRGATGGNYTAGG
cox1 P1 GAGGGGGGGACCCAATTCTCTTTCAATATT P1–P2: 3173
cob P2 GAAATGATAGGAATCGTGTTAGTGTGGGAT
cob P3 ATCCCACACTAACACGATTCCT P3–P4: 5853
cox1 P4 ACATAGTGGAAGTGGGCAACAACAT
cox1 P9 CTATGTTGTTGCCCACTTCCACTATGTTC P9–P10: 8098
cox1 P10 CCCCCTCCAGATGGATCAAAGAATGAAGTT
rrnL P5 TTAATTCAACATCGAGGTCGCAATCACAAG P5–P6: 2099
nad5 P6 TGGAAGCCCCATTATTGAGCAT
nad5 P7 CTGGTGTGTATCTCCTCTATCG P7–P8: 6244
rrnL P8 CTTGTGATTGCGACCTCGATGTTGAATTAA
nad4 P11 TTATGTTCGTGTCAAAACCCG P11–P12: 7394
nad4 P12 TAGTCGTTCATACTGTTTCCCTC
cob qcobF TTGGGGTCGCTTCTCGGGCT qcobF-qcobR: 201
cob qcobR TCCGACATGGGCGTAGCAGA
nad1 qnad1F TAGTATTATCCTTCTTTATTGGC qnad1F- qnad1R: 157
nad1 qnad1R ACAATGGTTAGGGGGAGGTAGAT
Nuclear gene β-ActinS CACGGTATCGTCACCAACTG β-ActinS-β-ActinA: 207
β-ActinA AGACAATACGGCTTGGATGG