Table 3. Real-time PCR results showing the relative copy number of mt chromosome I to chromosome II.

DNA	Replicate	Cob (Ct)	nad1 (Ct)	β-Actin (Ct)	Ratio A	Ratio B	Ratio C
SDS	1	19.08±0.014	19.95±0.057	23.38±0.050	19.698	10.778	1.828
	2	17.90±0.113	18.74±0.106	21.40±0.014	11.314	6.320	1.790
	3	18.30±0.064	19.27±0.035	22.90±0.092	24.251	12.381	1.959
Kit	1	17.76±0.085	18.65±0.156	20.87±0.028	8.634	4.659	1.853
	2	17.90±0.021	18.88±0.424	21.12±0.007	9.318	4.724	1.972
	3	17.92±0.050	18.78±0.014	21.72±0.778	13.929	7.674	1.815

Ct refers to the threshold cycle; Ratio A = 2^{−[Ctcob-Ctβ-Actin]}; Ratio B = 2^{−[Ctnad1-Ctβ-Actin]}; Ratio C = Ratio A/Ratio B = chromosome I/chromosome II; Kit: the total DNA was extracted using DNeasy Tissue kit (QIAGEN).