Skip to main content
. 2012 Mar 30;7(3):e33916. doi: 10.1371/journal.pone.0033916

Figure 3. UM-reconstructions of isolated hippocampi of a thy-1 EGFP-M mouse.

Figure 3

DBE clearing provides improved fluorescence and visibility of details. A: left hippocampus dehydrated with THF and cleared with BABB. B: corresponding right hippocampus dehydrated with THF and cleared with DBE. A1–B1: Olympus objective XLFluar 4×, N.A. 0.28, scale bar 100 µm. (A2–B2) 4× Olympus objective XLFluar 4×, N.A. 0.28, scale bar 20 µm. A3–B3: 4× Olympus objective XLFluar 4×, N.A. 0.28, scale bar 10 µm. CA1: cornu ammonis region one. gl: granular cell layer py: pyramidal cells.