Figure 7. Nuclear DNA damage in the spermatozoa of infertile men who carried c.474A/A.

(A.–C.) The spermatozoa were stained with AO (A.), TB (B.) and AB (C.) dyes. (A.) The spermatozoa with normal (green) or abnormal nucleus (yellow). (B.–C.) Spermatozoa with normal (light blue) or abnormal (dark blue) nucleus. (Magnification: ×1,000). (D.) Quantification of the percentage of AO-, AB- or TB- stained spermatozoa. At least 100 spermatozoa cells were counted in each case (*: p<0.05; Mann-Whitney test).