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. 2012 Mar 30;7(3):e32802. doi: 10.1371/journal.pone.0032802

Figure 3. Pharmacologic inhibition of serine/threonine phosphatases increases pS137-PFN1 in cultured cells.

Figure 3

A, HEK293 cells were treated with okadaic acid (OA) (a–d) or endothall (e–h) for 16 hr at increasing concentrations (1, 3 and 10 nM for OA; 1, 3 and 10 µM for endothall), followed by immunostaining with P3490 (green) and counterstaining with DAPI (blue). The number of P3490-positive cells and overall P3490 staining intensity increased notably at 10 nM of OA and 10 µM of endothall treatment. B, effects of OA and endothall on P3490 staining were quantified using a fluorescence plate reader. P3490 levels were normalized vs. DAPI, and the effects of the drugs were represented as relative change vs. the vehicle controls. OA and endothall increased P3490 staining dose-dependently. Error bars represent the SEM. Data are mean ± SEM of three independent experiments. C, HEK293 cells treated with 10 nM OA (a–b) or 10 µM endothall (c–d) were immunostained for total PFN1 (green) and counterstained with DAPI (blue). Merged images showed no changes in total PFN1 levels as a result of the drugs.