Figure 4. Inhibition of colony formation of SPC-A1 cells by NJ001 in soft agar. (×100).

The cell suspensions (2×10⁴ cells) mixed with 0.3% agarose and different concentrations of NJ001 or MCA2849 were layered on the top of culture media in 6-well culture plates and allowed to grow for 2 weeks before colonies were counted. Representative contrast images were shown. (A) 0 µg/mL NJ001, (B) 100 µg/mL NJ001, (C) 200 µg/mL NJ001, (D) 400 µg/mL NJ001, (E) 0 µg/mL MCA2849, (F) 100 µg/mL MCA2849, (G) 200 µg/mL MCA2849, (H) 400 µg/mL MCA2849.