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. 2012 Mar 30;7(3):e33453. doi: 10.1371/journal.pone.0033453

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Halsall et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Chromatin immunoprecipitation analysis of histone modifications on two VPA-responsive genes: KIF3C and RERE. Graphs indicate the enrichment of histone modifications at specific primer pairs in untreated cells (Control), and after eight hours of VPA treatment (+VPA). Histone modification enrichment is shown as a bound∶unbound ratio. The primers used are indicated on gene diagrams, which also indicate the transcription start site (TSS), exons (Empty boxes), and untranslated regions (Filled boxes). The abundance of a histone modification (‘Enrichment’) is calculated from the ratio of material detected in bound∶unbound fractions.