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. 2012 Mar 30;7(3):e33453. doi: 10.1371/journal.pone.0033453

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Halsall et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Chromatin-immunoprecipitation analysis of H4K8ac on: (A), Up-regulated gene (DLK1), (B), Down-regulated genes (LMO2, MYC) or (C), repetitive elements (ALU) in response to VPA treatment (5 mM). Analysis was performed on either untreated cells (Control), or during VPA treatment (15, 60 & 240 minutes). Primer locations are indicated on the gene diagrams. The abundance of a histone modification (‘Enrichment’) is calculated from the ratio of material detected in bound∶unbound fractions.