Table 1. Plasmids used in this study.
Plasmid | Resistance | Relevant genotype | Origin |
pSYM1 | None | mcsS, mcsI, mcsA, mcsB | This work |
pST76–A | Ampr | oriTS (30°C) | [43] |
pSYM1–ST76An | Ampr | mcsS, mcsI, mcsA, mcsB | This work |
pAZ6 | Ampr | mcsS, mcsI, mcsA, mcsB | This work |
pAZ8 | Ampr | mcsI, mcsA, mcsB | This work |
pAZ9 | Cmr | mcsS | This work |
pAZ10 | Cmr | ORF1 | This work |
pAZ11 | Cmr | ORF2 | This work |
pAZ12 | Cmr | mcsS 193–363 | This work |
pAZ13 | Ampr | mcsI | This work |
pAZ14 | Ampr | mcsI 361–651 | This work |
pGS1 | Ampr | PBAD | This work |
pAZ15 | Ampr | PBAD, mcsS | This work |
Ampr = ampicillin resistance; Cmr = chloramphenicol resistance; ORF = open reading frame; PBAD = araC PBAD activator-promoter.