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. 2012 Mar 30;7(3):e34039. doi: 10.1371/journal.pone.0034039

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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The genetic locus carrying the Mu insertion, indicated by an arrowhead, is depicted for each CHI strain. The genome annotations of the disrupted genes (shaded) are shown. Arrows refer to primer locations used to amplify and clone each locus for complementation analysis. Expression of folD and pssA were driven by the nptII promoter.