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. 2012 Mar 30;7(3):e34039. doi: 10.1371/journal.pone.0034039

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pAH83, which constitutively expresses YopM-Bla, was introduced into each mutant and the KIM5 parent, and the strains were evaluated by performing secretion assays as described in Figure 3. Proteins were visualized by immunoblotting with antibodies to β-lactamase (YopM-Bla), YopM, YopE (TTSS secreted proteins), YscD, YscF (TTSS apparatus components) Ail (outer membrane protein) and RpoA (cytoplasmic protein, loading and fractionation control). An asterisk indicates truncated Yops due to degradation by Pla protease.