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. 2012 Mar 30;7(3):e34039. doi: 10.1371/journal.pone.0034039

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Y. pestis strains KIM5 and CHI 1800 (rfaL ⁻) were subcultured into DMEM with or without EGTA and grown at 26°C for 2 hours and 37°C for 3 hours, followed by TCA precipitation of supernatant and cell pellet fractions and immunoblotting for early Yops (YopR, YopD, LcrV) and late Yops (YopH, YopM, YopE).