Figure 3. Northern blot analyses for SACOL0678 (A) and SACOL2176 (B) operon.

Total RNA was isolated from S. aureus COL at 0 (t₀), 10 (t₁₀), and 40 (t₄₀) min after the addition of amicoumacin A. 10 µg of total RNA isolated from each culture was separated in a formaldehyde-agarose gel and the RNA-blotted membrane was hybridized with SACOL0678- or SACOL2173(asp23)-specific digoxigenin-labeled probes. The sizes of the transcripts were determined by comparison to an RNA ladder on the same gel. The corresponding stained gels are shown underneath each blot. Schematic views of the gene loci based on NCBI COL genome site are shown with predicted transcripts. σ^B indicates locations of σ^B-controlled promoters. Microarray results of each operon's genes are summarized in the right panel of the corresponding Northern blot gels. Closed squares and open squares show samples taken at t₁₀ and t₄₀, respectively. The average of triplicates and standard deviations are indicated.