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. 2012 Mar 30;7(3):e34055. doi: 10.1371/journal.pone.0034055

Figure 3. ELISA confirms CBMC antiviral chemokine release following antibody-enhanced dengue virus infection or polyI:C exposure.

Figure 3

CBMCs, HMC-1 and KU812 cells were infected with dengue virus in the presence of a subneutralizing concentration (1∶10,000) of dengue-immune sera (DEN+Ab) or were exposed to 10 µg/mL polyI:C (pI:C (10)) or 1 µg/ml polyI:C complexed to Lipofectamine 2000 (pI:C (1)+Lpf). Controls included mock treatment, dengue virus only (DEN) and UV-inactivated dengue virus with dengue-immune sera (UV-DEN+Ab). After 24 hours, cell supernatants were harvested and measured for CCL4, CCL5 and CXCL10 by ELISA. Graphs show the mean ± SEM of 7–16 separate experiments. *P<0.05; **P<0.01; ***P<0.001.