Figure 4. Time course analysis reveals rapid production of CCL4 and CXCL10 by antibody-enhanced dengue virus-infected CBMCs.
CBMCs, HMC-1 and KU812 cells were infected with dengue virus with a subneutralizing concentration (1∶10,000) of dengue-immune sera (Dengue+Ab) for 12, 24, or 48 hours. Controls included mock treatment, dengue virus only (Dengue) and UV-inactivated dengue virus with dengue-immune sera (UV-Dengue+Ab). Cell supernatants were harvested and measured for CCL4, CCL5 and CXCL10 by ELISA. Graphs show the mean ± SEM of 3–16 separate experiments. *P<0.05; **P<0.01, ***P<0.001.