Figure 2 .

Characterization of Ypa1p and Ypa2p. (A and B) Strains expressing Ypa1p-HA or Ypa2p-HA were synchronized as described in Materials and Methods. Protein samples were prepared at the indicated times (minutes) after release from the block. Western blots were probed with either 12CA5 (HA), or TAT-1 (α-tubulin) as indicated. Cell cycle progression data are represented by the percentage of septated cells, indicated at each time point. (C) Proteins were prepared from strains expressing Ypa1-HA (lane 2), Ypa2-HA (lane 3), or both alleles (lane 1). Serial dilution of the sample indicates that the Ypa2p-HA signal in lane 1 is at least 30 times stronger than the Ypa1p-HA signal (Figure S2A). (D) Cells of the indicated genotype were grown to exponential phase and GFP was visualized as described in Materials and Methods. Bar, 10 μm.