| pCYC184 |
Cloning vector CmR TcR
|
Chang and Cohen (1978) |
| pYIplac211 |
Cloning vector, integrative vector URA3 ApR
|
Gietz and Sugino (1988) |
| pMB11 |
Cloning vector, sacB counterselection CmR
ori p15A |
Lab collection |
| pGRB2.0 |
Cloning vector, pRS406 URA3 C.g.CEN ARS ApR
|
De Las Peñas et al. (2003) |
| pRS306 |
Cloning vector, integrative vector URA3 ApR
|
Sikorski and Hieter (1989) |
| pAP599 |
Cloning vector for construction of knockout mutants with two FRT direct repeats flanking the hygromycin cassette to remove the selection marker for construction of multiple mutants in C. glabrata. [FRT-PPGK::hph::3′UTRHIS3-FRT] URA3 HygR ApR
|
Domergue et al. (2005) |
| pAP640 |
Cloning vector, pRS306 cut with NdeI/NaeI blunt ended with Klenow and religated. ura3Δ ApR
|
This work |
| pAP353 |
A 0.738-kb EcoRI/SalI PCR fragment carrying GFP (no BstXI site) and a 0.397-kb XhoI/KpnI PCR fragment carrying HIS3 3′UTR were cloned into pGRB2.0. For GFP promoter fusions. URA3 ApR. (Figure 1D) |
This work |
| pAP354 |
A 2.5-kb BstXI PCR fragment (primers 666/667) carrying the promoter region of EPA1 was cloned into pAP353. URA3 ApR PEPA1::GFP::3′UTRHIS3 (Figure 1D) |
This work |
| pAP385 |
A 3.1-kb XhoI PCR fragment (primers 723/724) carrying the 3′UTREPA1 fragment cloned at XhoI site of pAP354. URA3 ApREPA1Promoter::GFP::3′UTREPA1-3.1Kb (Figure 1D) |
This work |
| pAP407 |
Similar to pAP354 but 3′-UTR from EPA1.URA3 ApR PEPA1::GFP::3′UTREPA1-0.300Kb
|
Domergue et al. (2005) |
| pSP7 |
A 0.100-kb XhoI PCR fragment (primers 780/795) carrying the 3′ intergenic region of EPA1cloned into pAP407 digested with XhoI. URA3 ApR (+400 bp; Figure 2, line 4) |
This work |
| pSP19 |
A 0.50-kb XhoI PCR fragment (primers 780/841) carrying the 3′ EPA1 (NE mapped) was cloned into pAP407 digested with XhoI. URA3 ApR (+350 bp; Figure 2, line 5) |
This work |
| pSP20 |
A 0.150-kb XhoI PCR fragment (primers 780/842) carrying the 3′ intergenic region of EPA1cloned into pAP407 digested with XhoI. URA3 ApR (+450 bp; Figure 2, line 3) |
This work |
| pSP21 |
A 0.200-kb XhoI PCR fragment (primers 780/843) carrying the 3′ intergenic region of EPA1cloned into pAP407 digested with XhoI. URA3 ApR (+500 bp; Figure 2, line 1) |
This work |
| pSP26 |
A 0.200-kb XhoI/SalI PCR fragment (primers 776/839) carrying the 3′ UTR of EPA1cloned into pAP407 digested with XhoI/SalI. URA3 ApR (+200 bp; Figure 2, line 2) |
This work |
| pSP31 |
A 0.250-kb XhoI PCR fragment (primers 843/913) carrying the 3′ intergenic region of EPA1cloned into pSP26 digested with XhoI. URA3 ApR (−50 bp; Figure 2, line 7) |
This work |
| pSP32 |
A 0.200-kb XhoI PCR fragment (primers 780/843) carrying the 3′ intergenic region of EPA1cloned into pSP26 digested with XhoI. URA3 ApR (−100 bp; Figure 2, line 8) |
This work |
| pSP33 |
A 0.150-kb XhoI PCR fragment (primers 914/843) carrying the 3′ intergenic region of EPA1cloned into pSP26 digested with XhoI. URA3 ApR (−150 bp; Figure 2, line 9) |
This work |
| pSP34 |
A 0.100-kb XhoI PCR fragment (primers 915/843) carrying the 3′ intergenic region of EPA1cloned into pSP26 digested with XhoI. URA3 ApR (−200 bp; Figure 2, line 10) |
This work |
| pSP35 |
A 0.50-kb XhoI PCR fragment (primers 916/843) carrying the 3′ intergenic region of EPA1cloned into pSP26 digested with XhoI. URA3 ApR (−250 bp; Figure 2, line 11) |
This work |
| pSP38 |
Similar to pAP354 with the NE cloned immediately after the HIS3 3′-UTR. URA3 ApR PEPA1::GFP::3′UTRHIS3::NE+. |
This work |
| pSP105 |
A 0.300-kb fragment (primers 1135/1136) carrying the NE cloned 5′ the EPA1 promoter in pSP26. URA3 ApR (Figure 1D) |
This work |
