Figure 3 .

emb-9(tk75) and gon-1(e1254) suppress the reduced gonadal BM localization of EMB-9 in fbl-1(tk45) mutants. Localization of wild-type EMB-9 in wild type (A and B) and in the mutants fbl-1(tk45) (C and D), fbl-1(tk45); gon-1(e1254) (G), and gon-1(e1254) (H). Localization of mutant EMB-9 in emb-9(tk75) (E) and emb-9(tk75); fbl-1(tk45) (F) animals. Cross-sections of L3 (A and C) and L4 (B and D–H) hermaphrodites were stained with anti–EMB-9 (magenta) and DAPI (blue) and analyzed with confocal microscopy. Borders of gonads and intestines are illustrated below the photos. Patterns of EMB-9 localization to the basement membrane are indicated by thick (normal) and dotted (weak and discontinuous) magenta lines. We observed similar results in more than three experimental sets (30–40 sections were observed for each genotype in an experiment). Bars, 25 µm. i, intestine; g, gonad.