Table 4. Mitochondrial Respiration.
Control | DHA | ARA | DHA+ARA | |
Pyruvate+Malate | ||||
State 3 | 225±17 | 239±22 | 245±21 | 236±19 |
State 4 (−oligomycin) | 51.6±2.0 | 51.3±3.1 | 47.3±2.6 | 49.7±3.7 |
State 4 (+oligomycin) | 36.8±4.1 | 44.8±4.3 | 33.7±3.6 | 41.1±4.3 |
RCR (−oligomycin) | 4.5±0.4 | 4.7±0.4 | 4.8±0.2 | 5.2±0.4 |
RCR (+oligomycin) | 6.8±0.8 | 5.8±0.6 | 8.1±0.9 | 6.2±0.6 |
Palmityl-CoA+Carnitine+Malate | ||||
State 3 | 221±16 | 203±14 | 200±14 | 196±13 |
State 4 (−oligomycin) | 45.6±2.9 | 53.4±5.0 | 46.0±2.6 | 46.6±2.9 |
State 4 (+oligomycin) | 40.9±3.8 | 41.2±4.7 | 36.4±2.8 | 42.9±4.3 |
RCR (−oligomycin) | 5.0±0.3 | 4.1±0.4 | 4.3±0.3 | 4.5±0.4 |
RCR (+oligomycin) | 5.7±0.4 | 6.4±1.7 | 5.8±0.5 | 5.1±0.6 |
Succinate+Rotenone | ||||
State 3 | 392±16 | 398±19 | 360±22 | 393±25 |
State 4 (−oligomycin) | 122.7±3.6 | 120.1±7.3 | 111.6±4.7 | 108.2±6.9 |
State 4 (+oligomycin) | 107.8±4.9 | 123.9±7.8 | 100.0±8.3 | 123.6±7.0 |
RCR (−oligomycin) | 3.2±0.1 | 3.4±0.2 | 3.7±0.2 | 3.2±0.2 |
RCR (+oligomycin) | 3.7±0.1 | 3.3±0.1 | 3.8±0.3 | 3.2±0.1 |
Data are the mean ± SEM. CTRL, n = 14. DHA, n = 13. ARA, n = 13. DHA+ARA, n = 14. All Rates are expressed in ng atoms O·mg−1·min−1. The RCR, defined as the ratio of State 3 to State 4 respiration rate, was calculated from the State 4 rate with oligomycin.