Figure 5. Bortezomib treatment reduces IFN-γ production by CD4⁺ and CD8⁺ T cells in mesenteric lymph nodes during DSS-induced colitis.

IFN-γ production by mesenteric lymph node CD4⁺ (A) or CD8⁺ (B) T cells 7 days following DSS administration as determined by intracellular cytokine staining with flow cytometry analysis. Bar graphs indicate mean (± SEM) percentages and numbers of draining lymph node IFN-γ-producing CD4⁺ or CD8⁺ T cells following bortezomib or control treatment in one representative experiment with three mice per group. Significant differences between PBS-treated mice versus other group are indicated; *P<0.05, **P<0.01. Similar results were obtained in at least two independent experiments.