Fig. 1.

Minimalistic proteomics setup. Yeast samples were lysed and prepared by the FASP method. Peptides were purified on StageTips and placed in an autosampler, which loads them directly on to a relatively long column (50 cm). The binary gradient system is provided by an UHPLC system (EASY nLC 1000) system coupled to a bench top quadrupole Orbitrap mass spectrometer (Q Exactive) via a nanoelectrospray source. The data obtained were analyzed in the MaxQuant computational proteomics platform, and bioinformatics analyses were performed using the Perseus tool.