Fig. 2.

In-depth coverage of the yeast proteome. A, number of peptides identified in individual runs with and without matching between the runs. Peptides identified by matching are indicated in green. B, proteins identified in individual runs with the gain from matching between the runs indicated in green. Proteins identified with single peptide hits are shown in red. C, the median sequence coverage of individual runs after matching was ∼17%. The median sequence coverage from the combined run for 4099 proteins was 22.9% as shown. D, the conjoint circles represent the frequency of identification of proteins in the six runs. Proteins identified in all six runs were designated as core proteome in the innermost circle.