Fig. 4.

Biological reproducibility of model selection and calibration curve generation. A and B, to test the reproducibility of the determined mean fold error distributions based on varying peptide and transition combinations, we performed the Monte Carlo cross-validation analysis on three biological L. interrogans samples: a control sample (Fig. 3A), 12 h of treatment with ciprofloxacin (A) and 24 h of treatment with ciprofloxacin (B). C, overlay of linear calibration curves generated for the three different biological samples over a measurement period of 70 h. Each data point represents an averaged value of three technical replicates.