Table I. Absolute protein abundance levels of 16 anchor point proteins using stable-isotope dilution LC-MS.
| Gene namea | Protein identificationa | Peptide sequence | Control copies/cell |
Ciprofloxacin copies/cell |
||||
|---|---|---|---|---|---|---|---|---|
| 12 h |
24 h |
|||||||
| Peptidesb | Proteinsc | Peptidesb | Proteinsc | Peptidesb | Proteinsc | |||
| clpB | YP_001955.1 | AVDLIDEASSK | 615 ± 2 | 615 ± 2 | 376 ± 6 | 376 ± 6 | 595 ± 2 | 595 ± 2 |
| clpP2 | YP_001896.1 | IAEVFEELTGSK | 2061 ± 24 | 2857 ± 1126 | 1637 ± 15 | 2299 ± 937 | 1802 ± 22 | 2508 ± 999 |
| clpP2 | YP_001896.1 | IFLWGPVTDESSK | 3653 ± 29 | 2962 ± 20 | 3214 ± 65 | |||
| fas1 | YP_003223.1 | AIAEEVPK | 1107 ± 6 | 1431 ± 458 | 1099 ± 40 | 1409 ± 441 | 1112 ± 14 | 1436 ± 459 |
| fas1 | YP_003223.1 | EFFDTSFK | 1755 ± 14 | 1719 ± 7 | 1761 ± 2 | |||
| flgE | YP_001158.1 | VATAVFNNPAGLDK | 87 ± 3 | 87 ± 3 | 113 ± 5 | 113 ± 5 | 73 ± 2 | 73 ± 2 |
| fliF | YP_001355.1 | IISDFEEDLEK | 80 ± 4 | 103 ± 33 | 80 ± 0 | 85 ± 6 | 92 ± 6 | 99 ± 12 |
| fliF | YP_001355.1 | GFTPDGPAGTEPNIAPGYK | 127 ± 0.8 | 89 ± 1 | 106 ± 2 | |||
| groL | YP_001299.1 | VEDALSATR | 10265 ± 15 | 11364 ± 1555 | 10985 ± 299 | 12302 ± 1886 | 12621 ± 202 | 14179 ± 2213 |
| groL | YP_001299.1 | AVTAAVESIQK | 12463 ± 42 | 13619 ± 187 | 15737 ± 34 | |||
| groS | YP_001300.1 | VGDTVLYGK | 8707 ± 16 | 16451 ± 10951 | 6640 ± 100 | 12583 ± 8406 | 6581 ± 28 | 12179 ± 7916 |
| groS | YP_001300.1 | ESDILAVVK | 24195 ± 120 | 18526 ± 390 | 17776 ± 214 | |||
| hslU | YP_001559.1 | LLEEVSFEGPDLPESQR | 395 ± 8 | 395 ± 8 | 434 ± 4 | 434 ± 4 | 437 ± 6 | 437 ± 6 |
| hsp15 | YP_002146.1 | DVQVQLEK | 807 ± 19 | 807 ± 19 | 3085 ± 76 | 3085 ± 76 | 2432 ± 59 | 2432 ± 59 |
| LIC_10600 | YP_000584.1 | TEVITHANLVR | 2151 ± 9 | 2151 ± 9 | 1665 ± 77 | 1665 ± 77 | 1614 ± 7 | 1614 ± 7 |
| LIC_11890 | YP_001839.1 | ANLGAYFNR | 21995 ± 119 | 21995 ± 119 | 17607 ± 76 | 17607 ± 76 | 20233 ± 148 | 20233 ± 148 |
| LIC_12210 | YP_002145.1 | ILELPTEVDSEK | 723 ± 3 | 723 ± 3 | 2086 ± 21 | 2086 ± 21 | 1889 ± 6 | 1889 ± 6 |
| mreB | YP_001227.1 | TGGDEFDEAIIK | 1989 ± 22 | 1989 ± 22 | 2164 ± 20 | 2164 ± 20 | 2520 ± 10 | 2520 ± 10 |
| recA | YP_001697.1 | IVEIYGPESSGK | 697 ± 5 | 697 ± 5 | 3810 ± 45 | 3810 ± 45 | 4154 ± 11 | 4154 ± 11 |
| rpoB | YP_000733.1 | TFDLGEVGR | 2378 ± 16 | 2378 ± 16 | 2376 ± 34 | 2376 ± 34 | 2594 ± 43 | 2594 ± 43 |
| rpoC | YP_000734.1 | FATSDLNDLYR | 5027 ± 4 | 5027 ± 4 | 5355 ± 232 | 5355 ± 232 | 6010 ± 50 | 6010 ± 50 |
a Gene names and protein identifiers were extracted from NCBI genome database numbers NC_005823 and NC_005824.
b Endogenous peptide concentrations were determined by spiking of SIS peptides; the given standard deviations were calculated based on technical triplicates.
c For proteins quantified with two peptides, the abundance level and standard deviation were calculated from the average of both peptide values.