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Peptide ratios along the PrESTs sequences. The PrEST master mix was spiked into a lysate of HeLa cells and measured against the endogenous protein. The peptide ratios were extracted to quantify the proteins. The variation of the peptide ratios along the sequence is depicted. Overlapping peptides are because of missed cleavages. The gray bars correspond to the predicted limit tryptic peptides for the PrEST region.
