Fig. 2.

Selenoprotein mRNA expression in Caco-2 cells during Se depletion. a Caco-2 cells were grown for 3 days in either Se-depleted or Se-supplemented (40 nM) medium, RNA extracted and mRNA levels assessed by semi-quantitative RTPCR. Values were normalised to GAPDH and calculated relative to the expression in the Se-supplemented group. Expression of GPX1, SELH and SELW was significantly lower in the Se-depleted cells, but expression of GPX2 and GPX4 was unaffected. *P < 0.05, **P < 0.01. b Western blotting of total cell protein from cells grown for 3 days in either Se-depleted or Se-supplemented medium. Note that the levels of GPx1 and GPx4 are both undetectable in Se-depleted cells