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Effects of Se depletion on interleukin 8 expression. a Semi-quantitative RTPCR amplification of NF-κB target gene IL8 and housekeeping gene GAPDH. Cells were grown in Se-depleted or 40 nM Se-supplemented culture medium (3 days) and either stimulated with TNFα for 1 h or treated with carrier. b Quantification of IL8 mRNA levels in cells grown in Se+ and Se− media, normalised against GAPDH and related to expression in the Se-supplemented cells. ***P < 0.001
