Fig. 1.

PL-cultured ASC have a short population doubling time and a fast attachment rate. a Population doubling time (PD, in days) shown for FBS- and PL-cultured ASC from passages 1–3. Bars means ± SD. FBS: n = 6, PL: n = 5; ***p  < 0.001. b Attachment rate of FBS- and PL-cultured cells to tissue culture plastic, shown as percentage of seeded cells at 5, 10, 20, 30, 60, 120, and 180 min and 24 h (1,440 min). Bars means ± SD. FBS: n = 6, PL: n = 5; **p  < 0.01, ***p  < 0.001. c Attachment rate of FBS- and PL-cultured ASC to HUVEC, shown as a percentage of seeded cells at 10 and 60 min. Bars means ± SD. n = 4; *p  < 0.05