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. 2012 Mar 13;3:738. doi: 10.1038/ncomms1744

Figure 5. Pharmacological treatment with DIOA affects GABAergic currents and regulates the expression of α3-α1 and δ subunits.

Figure 5

(a) Example of I–V relations of isoguvacine (10 μM) evoked GABAA currents at increasing holding potentials in control neurons. (b) Example of I-V relations of isoguvacine (10 μM) evoked GABAA currents at increasing holding potentials in DIOA treated neurons. (c) Summary of ECl at DIV12 in control (grey bar n=8) and DIOA treated neurons (white filled bar n=8; *P<0.05; unpaired t-test). (d) Representative sIPSCs from control and DIOA (10 μM) treated neurons. (e) Summary of sIPSCs decay kinetics in control (grey bar n=33) and DIOA treated neurons (white filled bar n=35; ***P<0.001; unpaired t-test). (f) Summary of sIPSC frequency in control (grey bar n=33) and DIOA treated neurons (white filled bar n=35; ***P<0.001; unpaired t-test). (g) Summary of tonic current amplitude in control (grey bar n=25) and DIOA treated neurons (white filled bar n=32; ***P<0.001; unpaired t-test). (h) Quantification of immunofluorescence signals for α3 and α1 subunits (α3 control, grey bar n=52; DIOA, white filled bar n=55; *P<0.5; unpaired t-test; α1 control, grey bar n=53; DIOA, white filled bar n=59; ***P<0.001; unpaired t-test). (i) Quantification of immunofluorescence signals for δ subunit in control (grey bar n=71) and DIOA treated neurons (white filled bar n=41; ***P<0.001; unpaired t-test). (j) Somatic immunostaining of immunofluorescence signals for α3 and α1 subunits in control and DIOA treated neurons at DIV12. (k) Somatic immunostaining of immunofluorescence signals for δ subunit in control and DIOA treated neurons. Scale bar: 23 μm. Data is presented as mean±s.e.m.